How To Use Our PRP For Platelet Separation:
In a 20 cc syringe take 2 cc of anticoagulant, then draw 18cc of blood from a subject (final volme : 20 cc).
Inject the drawn blood to the PRP kit through the upper injection port until the blood level reaches the 20cc scale marked on the kit. (For easier injection, tilt the kit)
- Separate the plasma layer and red blood cell (RBC) layer by the first centrifugation. (Prior to centrifugation, balance the weight of kit with counterbalance)
- Conditions for the First Centrifugation (RPM) Female - 3,000 RPM 3 minutes Male - 3,000 RPM 4 minutes When fail the seperation - 3,000 RPM 1 minutes *For subjects taking aspirin, antihypertensive drugs, with cardiac disease, anemia, or during menstruation, the centrifugation should be done for 4 min at 1,000 RCF.
After the first centrifugation, identify the separation position of the plasma and the RBC layer. Then adjust the height of the separated boundary to the indicated point by pushing up or pulling down the adjusting knob located at the lower part of the Kit. (If the volume of the plasma layer or the separation is not quite well done, additional centrifugation may be done for about 1-2 min at same RPM)
In order to block the plasma and the RBC layer completely, fasten the adjusting knob (counter-clockwise) located at the lower part of the Kit. Then fasten the valve(counter-clockwise) just above the adjusting knob. Finally fasten the adjusting knob(counter-clockwise) again.
Put the fastened PRP Kit to the centrifuge with counterbalance for the second centrifugation to enrich the concentrated platelet. *Condition for the second centrifugation : 6 min at 3,200 RPM
Place the PRP Kit in its upright position with care in order to prevent mixing of the separated PRP and PPP. Open the upper sillicone lid on the Kit. Using a 10cc syringe with a long needle (> 5 cm) take out the PPP layer slowly from the upper part leaving 4 cc at the base.
Shake and mix the remaining 4 cc of the PRP layer. Then administrate the PRP.